By Iliades, Chris, MD 
January 01, 2018


Does this test have other names?

Progesterone blood test, serum progesterone

What is this test?

This test measures the level of a hormone called progesterone in your blood.

Your ovaries make progesterone after ovulation. The most important role of progesterone is to get your uterus ready so that it can receive, implant, and support a fertilized egg during pregnancy.

Progesterone levels are usually low during the first stage (follicular stage) of your menstrual cycle. Ovulation is called the luteal stage, when the egg is released into the fallopian tube. After ovulation, progesterone levels go up for about 5 days before going back down. If pregnancy happens, your progesterone levels will slowly rise from the ninth week of pregnancy until the 32nd week. The placenta will begin to make progesterone after 12 weeks to help your pregnancy stay healthy.

Because progesterone levels change according to the stage of your menstrual cycle and the stage of your pregnancy, this blood test may be repeated many times.

Why do I need this test?

You may need this test as part of a fertility study if you are having trouble getting pregnant. A progesterone blood test is the best sign of ovulation. If you are pregnant, you may have this test to check the health of your pregnancy.

You may have this test to find out if:

  • You are ovulating

  • Your ovaries are working the way they should

  • Your pregnancy is at risk

What other tests might I have along with this test?

Your healthcare provider may order other blood tests as part of a fertility study. Your provider may also order an ultrasound to measure the thickness of the lining of your uterus (endometrium).

If you are pregnant, your healthcare provider may order a blood test to measure a hormone called human chorionic gonadotropin, or hCG, to help determine if your pregnancy is at risk.

What do my test results mean?

Many things may affect your lab test results. These include the method each lab uses to do the test. Even if your test results are different from the normal value, you may not have a problem. To learn what the results mean for you, talk with your healthcare provider.

Test results may vary depending on your age, gender, health history, the method used for the test, and other things. Your test results may not mean you have a problem. Ask your healthcare provider what your test results mean for you. 

Progesterone levels vary based on when during your menstrual cycle you have it done, and whether you have reached menopause.

Progesterone is measured in nanograms per milliliter (ng/mL). Below are ranges that are considered normal: 

  • 0.1 to 0.3 ng/mL for prepubescent girls

  • 0.1 to 0.7 ng/mL in the follicular stage of the menstrual cycle

  • 2 to 25 ng/mL in the luteal stage of the menstrual cycle

  • 10 to 44 ng/mL during the first trimester of pregnancy

  • 19.5 to 82.5 ng/mL during the second trimester of pregnancy

  • 65 to 290 ng/mL during the third trimester of pregnancy

Other conditions can cause abnormal results of a progesterone blood test. For example:

  • Increased progesterone during pregnancy can mean that you have twins or an abnormal type of pregnancy called a molar pregnancy.

  • Increased progesterone when you are not pregnant could mean you have a type of ovarian tumor called a lipid ovarian tumor, or chorionepithelioma.

  • Decreased progesterone during pregnancy could mean that you have a risk for miscarriage (spontaneous abortion).

  • Decreased progesterone when you aren't pregnant could mean that you don't have enough female hormones, a condition called hypogonadism.

How is this test done?

The test is done with a blood sample. A needle is used to draw blood from a vein in your arm or hand. 

Does this test pose any risks?

Having a blood test with a needle carries some risks. These include bleeding, infection, bruising, and feeling lightheaded. When the needle pricks your arm or hand, you may feel a slight sting or pain. Afterward, the site may be sore. 

What might affect my test results?

Certain medicines, such as birth control pills or steroids, may affect your results.

How do I get ready for this test?

You don't need to prepare for this test. Let your healthcare provider know the date of your last menstrual period. Be sure your provider knows about all medicines, herbs, vitamins, and supplements you are taking. This includes medicines that don't need a prescription and any illicit drugs you may use.


January 01, 2018


DeMoranville V. The Gale Encyclopedia of Nursing and Allied Health. 2013, ed. 3, pp. 1997-3000., McPherson. Henry's Clinical Diagnosis and Management by Laboratory Methods. 2017, 23rd ed., pp. 402-04., Progesterone supplementation to reduce the risk of spontaneous preterm birth. UpToDate, Progesterone. Davis's Comprehensive Handbook Of Laboratory & Diagnostic Tests With Nursing Implications. Van Leeuwen AM. 2015, 6th ed., Progesterone: Drug information. UpToDate.

Reviewed By:  

Freeborn, Donna, PhD, CNM, FNP,Haldeman-Englert, Chad, MD